Environmental Project, 977 – Survey of Estrogenic Activity in the Danish Aquatic Environment

Survey of Estrogenic Activity in the Danish Aquatic Environment

8 Survey data

The table on the following pages lists all data obtained in the project. Each sample is represented by two lined sections where the two lines contain the results from measuring the concentrations estrogens with and without enzymatic deconjugation, respectively (i.e., free and total estrogens).

In the first column of the table, the name of the sampling site and the sample label is listed. The sampling label contains information on the sampling location, sample category, the sample type and the season for sampling. These groups are listed in the columns named “Sample Site”, “Category”, “Sample type”, and “Season”. The explanation of the different codes used under each of these columns is given after this text.

The last seven columns presents the results divided in two main groups. In the first main group the E2-equivalents are presented in three columns. The first column, “chemical”, contain E2-equivalents estimated assuming the following relative responses: E1=0.29, EE2=0.88 and E2=0.04. Measurements below the quantification level (i.e., typical below 0.1 ng/L) are not included in the chemical E2-equivalent estimation and not included in the statistical evaluation. The second column, “Biological”, contain the results using the YES-assay and the third column, “2^nd biological”, contain results from samples from the sample bank. The main group, “Chemical analysis”, contain the data obtained by analysing the samples with GC-MS/MS. Results are presented for each of the four analytes E1, E2, αE2, and EE2. In the columns presenting the results, a number of abbreviations are used. These are listed and explained in the following.

Five samples have been excluded: A1-08U (large difference free and total); B4-12U (large difference between chemical and biological measurement); G1-01X (sampling failure); G2-02X (effluent rather than influent sample); and L0-26O (large difference between chemical and biological measurement).

In the cases where the bank sample is analysed, it is the result of this (2^nd biological sample) that is used in calculations.

Abbreviations and codes used in the table are presented overleaf.

Category codes

A	WWTP MBND(C)	G	Septic tank effluent	M	Generally exposed streams
B	WWTP MBN(C)	H	Drain, manure treated field	N	Streams in husbandry areas
C	WWTP MB(C)	I	Drain, sludge treated field	O	Lakes in husbandry areas
D	WWTP M(C)	J	Stormwater retention basin	P	Reference streams
E	Reed bed	K	Aquaculture (fish farm)	Q	Reference lakes
F	Biological sand filter	L	Streams receiving WWTP effluents	R	Generally exposed lakes

Sample types

Effluent	WWTP effluent	Downstream 100 m	100 meters downstream of WWTP discharge point
Influent	WWTP influent	Downstream 10 m	10 meters downstream of WWTP discharge point
Miscellaneous	Samples where distinction between upstream/downstream or influent/effluent makes no sense(e.g. general sampling in streams)	Upstream	Upstream of the WWTP discharge point

Season codes

Winter	February – March
Spring	April – May
Summer	June – August
Fall	September – October

Measurement codes

n.d.	Not detected, only chemical
negative	Sample tested negative in biological analysis
[blank field]	Not analysed. Sample stored in sample bank
<0.1	Chemical specie detected under detectable concentration of e.g., 0.1 ng/L

Click here to see Survey data schemes.

8 Survey data

8.1 Explanations and abbreviations