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Bioremediering ved hjælp af planter og gensplejsede bakterier 5 Videregående litteratur
5.1 Publikationer fra projektet.Boldt, T.S. Kan bakterier rense jord ?. Specialhistorie i Forskningsforums og Forskningsrådenes årsberetning 2000. Boldt, T.S., U. Karlson, J. Sørensen, S. Molin and C. Ramos. In situ degradation and effect of 3- and 4-PCB on Ps. fluorescens F113 in the rhizosphere of alfalfa studied by using CLSM. In prep. Boldt, Tina S., Kirsten B. Lejbølle, Jan Sørensen and Ulrich Karlson. Evaluation of a genetically modified Ps. fluorescens F113 for phytoremediation of 4-PCB polluted soil. In prep. Dowling, D. (1999) Directed Evolution of bacteria for the detoxification of pollutants. The Irish Scientists Year Book 1999 p101 (ed. Mollam, C.) Sampton Ltd., Dublin. Karlson, U, Dowling, DN, O'Gara, F, Rivilla, R, Bittens, M, Francesconi, S, Pritchard, H and H.C. Pedersen (1998) . Development of self-contained plant/GMM systems for soil bioremediation. Proceedings Second CCRO Workshop. Past, Present and Future Risk Assessment when using GMO's. Gert E. de Vries (editor). ProBio Partners V.O.F., Overschild, The Netherlands, pp. 23-31 O'Riordan, B., R. Rivilla, M. Sheehan, J. Morrissey, F. O'Gara. Development of plant exudate inducible systems for in situ degradation of PCBs. In Preparation. Power, B and Dowling, DN. Long Term Survival, release and activity of Genetically Modified inoculant bacteria encapsulated in alginate beads. In preparation for Industrial Biotechnology. Sánchez-Contreras, M., Lloret, J., Martín, M., Villacieros, M., Bonilla, I. and Rivilla, R. (2000) PCR use of highly conserved DNA regions for identification of Sinorhizobium meliloti. Appl. Environ. Microbiol. 66:3621-3623. Sánchez-Contreras, María , Marta Martín, Marta Villacieros, Fergal O´Gara, Ildefonso Bonilla and Rafael Rivilla. Pseudomonas fluorescens F113 phase variants are affected in multiple traits relevant to rhizosphere colonization. Submitted to J. Bact. Sherlock, O., Whelan, C., Ryan,D and D.N. Dowling. Genetic and biochemical analysis of a mutant bphC allele from a GM pseudomonad. In preparation for FEMS Microbiology. Sherlock,O, Whelan,C., Ryan,D, Francesconi,S and Dowling,D. Development of a Real time PCR assay for the detection of a PCB degrading genetically modified organism in soil. Submitted to FEMS Microbiol. Ecol. Villacieros, M. et al. Design of expression systems based on rhizobial nod promoters for PCBs rhizodegradation. In Preparation. Villacieros, M., B. Power, M. Sánchez-Contreras, J. Lloret, R.I. Oruezabal, M. Martín, F. Fernández-Piñas, I. Bonilla, C. Whelan, David N. Dowling and R. Rivilla.Colonization behaviour of Pseudomonas fluorescens and Sinorhizobium meliloti in the alfalfa (Medicago sativa) rhizosphere. Submitted to Appl. Environ. Microbiol. Villacieros, M., Power,B , Sanchez-Contreral,M, Lloret,J, Oruezabal,R.I, Martin,Marta, Fernandez-Pinas, Whelan,C., Bonilla,I, Dowling, DN and Rivilla, R. Colonisation behaviour Pseudomonas fluorescens and Sinohizobium meliloti in the alfalfa rhizosphere. Submitted to Applied and Environmental Microbiology (2001). Ryan,D., Sherlock,O., Whelan,C., Francesconi,S., Rivilla, R. and Dowling,D. Location of the the bph cassette and its insertion site in the GM bacterium F113rifpcb. Submitted to FEMS Microbiol. Letters. 5.2 PhD-, Master- og speciale afhandlinger fra projektet.Boldt, Tina S. (2001). Rhizoremediation of PCBs by GM strains of P. fluorescens F113 inoculated to willow and alfalfa. PhD thesis. Gaillet. David (1999) "Bioremediation Research. Establishment of a Model-System: Quantification of the Roles Played by Some of the Biotic Participants in the Remediation of Chlorinated Benzoic Acids" Danisco Seed. MSc. Thesis. O'Riordan, Brid. Exploitation of plant exudate inducible gene promoters to develop Pseudomonas strains for biotechnological applications. In preparation. Msc Thesis. University College Cork, Ireland Power,B. (2001). Development of a practical delivery system for the storage, delivery and release of microbial inoculants for bioremediation applications. PhD thesis. Sánchez-Contreras, M. (2001). Variación de Fase de Pseudomonas fluorescens durante la colonización de la rizosfera de alfalfa. PhD Thesis. Universidad Autónoma de Madrid. (In Spanish). Villacieros M. (2000). Desarrollo de un sistema integrado planta/microorganismos modificados geneticamente para descontaminación. PhD. Thesis. Universidad Autónoma de Madrid (In Spanish). Whelan,C. (2001). Construction and analysis of GM bacteria with re-regulated expression of bph genes. PhD thesis. 5.3 Konferencebidrag fra projektet.Brazil,G.M., Bermingham,A., Gardiner,S., O’Sullivan,O., O’Gara,F. and Dowling,D.N. (1997) Design of bacterial inoculants for the degradation of polychlorinated biphenyls (PCBs) in the rhizosphere. Proceedings VI International Congress on Pseudomonas: Molecular Biology and Biotechnology p23. CSIC, Madrid. Brazil,G.M., Bermingham,A., Gardiner,S., Whelan,C., O’Gara,F., Karlson,U. and Dowling,D.N. (1997) Novel bacterial inoculants for the biodegradation of polychlorinated biphenyls (PCBs) in the rhizosphere. Society for General Microbiology (SGM) Symposium - Microorganisms: the answer to environmental pollution? P57 UCD, Dublin. Brazil,G.M., Kenefick,L., Dowling,D.N. and O’Gara,F. (1997) Quantification of lateral transfer of a novel degradative trait,bph, from a genetically modified rhizosphere pseudomonad, F113pcb, in the laboratory and in the sugar beet rhizosphere. Proceedings of the Society for General Microbiology p27, University of Limerick, Galway. Gilmartin, Niamh, David Ryan, Orla Sherlock, Donal Skelly and David Dowling. Rhodococcus erythropolis ITCBP; an isolate with potential for degradation of PCBs. Proceedings of the Society for General Microbiology, Institute of Technology Waterford, 2001. O'Riordan, B., Hogan, I., Sheehan, M.M., Carnicero, P. and O'Gara, F. (2000). Exploitation of Green Fluorescent Protein for the study of the behaviour of the Pseudomonas fluorescens strain F113 in the sugarbeet rhizosphere. Proceedings of SGM conference on Microbial Ecology, NUIG, O'Riordan, B., Sheehan, M. M., Carnicero, P. and O'Gara, F. (1999). Exploitation of plant exudate responsive promoters in the construction of inducible expression systems. Proceedings of SGM conference. Colerain, Northern Ireland. Power,B., Conway, S., and Dowling, D.N. (1998) Design of microbial inoculants for environmental applications. Procedings Science Research Colloquim. Institute of Technology Tallaght, Dublin. Sánchez-Contreras, M., Martín, M., Villacieros, M. and Rivilla, R. Phase variation in Pseudomonas fluorescens. Implications for Bioremediation. In Biotechnology for Environmental Applications.p T13. Roskilde 2000. Sánchez-Contreras,M., Villacieros,M., Fernández-Piñas, F., Lloret,J., Martín, M. Bonilla,I y Rivilla,R. Estudio del potencial biorremediador de Sinorhizobium meliloti. Res. VIII Reunión Nacional de Fijación de Nitrógeno. p. 189 (1998). Sheehan, M. M., O'Riordan, B. and O'Gara, F. (2000) Integrated Plant/GEM Systems for in situ Soil Bioremediation. Proceedings of EU conference: Biotechnology for Environmental Applications. Roskilde, Denmark. Sherlock, O., O'Shaughnessy, K. Gardiner, S. and D.N. Dowling. (1999) Generation of novel PCB degrading genes using molecular genetic techniques. Proceedings of the 4th Research Science Colloquium, Institute of Technology Letterkenny. Sherlock,O, and Dowling,D. (2000) Development of a PCR assay for the detection of a PCB degrading GEM in soil. In Proceedngs of SGM Conference on Microbial Ecology, NUIG, Galway. Villacieros, M., Sánchez-Contreras, M. Martín, M. and Rivilla, R. Use of rhizobial nod promoters for foreign gene expression in the alfalfa rhizosphere. In Biotechnology for Environmental Applications.p P13. Roskilde 2000. Villacieros, M., Sánchez-Contreras, M., Martín, M. and Rivilla, R. Expression systems based on rhizobial nod promoters can be used to express foreign genes in the alfalfa rhizosphere. Abs. 4th European Conference on Nitrogen Fixation. p. 216. Sevilla 2000. Villacieros,M., Sánchez Contreras,M., Lloret,J., Fernández-Piñas, F., Bonilla,I. y Rivilla, R. Efectos de la coinoculación de alfalfa con Sinorhizobium meliloti EFB1 y Pseudomonas fluorescens F113. Res. VIII Reunión Nacional de Fijación de Nitrógeno. p. 195-196 (1998). Whelan, C., Gardiner, S., O'Gara, F. and Dowling, D.N. (1998) Genetic Manipulation of a Genetically Modified Pseudomonas with Potential to Degrade Polychlorinated Biphenyls (PCBs). Procedings Science Research Colloquim. Institute of Technology Tallaght, Dublin. Whelan,C., Power,B., Sherlock,O., Ryan,D. and Dowling,D.N. (2000) Development of rhizosphere bacteria and delivery systems for the degradation of PCBs . Proceedings of EU Conference: Biotechnology for Environmental Applications, p15. Roskilde, Denmark.
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