| Front page | | Contents | | Previous | | Next |
Survey of chemical substances in kohl and henna products
4 Analyses results
4.1 Kohl products
4.1.1 Analysis results - screening
The analysis results from the screening of kohl products are listed in the tables below.
Table 1. Kohl products, Inorganic analysis – screening analyses
Click here to see Table 1
Table 1. continued
Analyte |
DL µg/g |
%RSD |
Sample no.: 31341- |
52 |
56 |
59 |
60 |
61 |
62 |
64 |
65 |
66 |
µg/g |
µg/g |
µg/g |
µg/g |
µg/g |
µg/g |
µg/g |
µg/g |
µg/g |
Li |
0.5 |
4.6% |
24 |
12 |
- |
1.2 |
- |
- |
- |
- |
- |
Be |
1 |
14% |
1.9 |
- |
- |
- |
- |
- |
- |
- |
- |
B |
5 |
4.6% |
9.2 |
- |
- |
34000 |
44 |
- |
- |
- |
9.1 |
Na |
1 |
1.9% |
350 |
43 |
70 |
2400 |
54 |
32 |
43 |
52 |
150 |
Mg |
1 |
1.7% |
4000 |
3300 |
15 |
830 |
27 |
13 |
21 |
17 |
120 |
Al |
1 |
4.7% |
12000 |
470 |
52 |
3500 |
95 |
58 |
38 |
30 |
190 |
Si |
10 |
6.3% |
2900 |
5000 |
70 |
360 |
170 |
99 |
110 |
95 |
600 |
K |
5 |
2.8% |
5700 |
290 |
16 |
6400 |
29 |
19 |
14 |
17 |
360 |
Ca |
5 |
2.8% |
170 |
130 |
70 |
110000 |
200 |
54 |
78 |
64 |
410 |
Sc |
0.5 |
4.1% |
1.9 |
1.8 |
- |
1.2 |
- |
- |
- |
- |
- |
Ti |
0.5 |
6.5% |
76 |
59 |
3.5 |
15 |
7.6 |
3.0 |
1.9 |
1.7 |
13 |
V |
0.5 |
4.4% |
0.86 |
- |
5.2 |
75 |
64 |
15 |
16 |
12 |
1.3 |
Cr |
0.5 |
2.2% |
3.2 |
8.2 |
13 |
3.6 |
1.6 |
0.88 |
2.2 |
1.5 |
1.1 |
Mn |
0.1 |
0.99% |
330 |
280 |
170 |
180 |
1.4 |
1.5 |
0.87 |
0.60 |
12 |
Fe |
10 |
8.2% |
150000 |
150000 |
56000 |
490 |
81 |
41 |
47 |
27 |
270 |
Co |
0.1 |
5.6% |
31 |
27 |
1.8 |
3.6 |
3.3 |
0.96 |
- |
- |
0.11 |
Ni |
0.5 |
4.4% |
39 |
41 |
18 |
56 |
54 |
21 |
13 |
9.9 |
1.4 |
Cu |
0.1 |
3.6% |
0.48 |
2.0 |
8.9 |
880 |
3.5 |
1.7 |
1.4 |
5.6 |
3.7 |
Zn |
0.5 |
6.8% |
16 |
12 |
12 |
120000 |
36 |
5.0 |
3.3 |
11 |
13 |
Ga |
0.1 |
8.0% |
5.1 |
0.21 |
0.38 |
0.81 |
0.13 |
- |
- |
- |
0.16 |
As |
1 |
17% |
- |
- |
- |
6.1 |
- |
- |
- |
- |
- |
Se |
1 |
29% |
- |
- |
- |
8.5 |
- |
- |
- |
- |
- |
Rb |
0.1 |
2.9% |
79 |
3.0 |
- |
- |
- |
- |
0.12 |
- |
0.83 |
Sr |
0.1 |
2.1% |
2.9 |
1.3 |
58 |
280 |
0.60 |
0.40 |
0.69 |
0.62 |
2.7 |
Y |
0.1 |
2.4% |
- |
0.29 |
- |
12 |
- |
- |
- |
- |
0.12 |
Zr |
0.1 |
11% |
7.2 |
18 |
0.27 |
0.83 |
0.43 |
0.18 |
- |
- |
- |
Nb |
0.1 |
3.1% |
3.7 |
- |
0.70 |
- |
- |
- |
- |
- |
- |
Mo |
0.1 |
4.9% |
0.39 |
0.28 |
0.14 |
0.92 |
0.57 |
- |
0.17 |
- |
0.19 |
Ru |
0.1 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Pd |
0.1 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Ag |
0.1 |
1.8% |
- |
- |
- |
- |
- |
- |
- |
- |
1.1 |
Cd |
0.05 |
12% |
- |
- |
0.06 |
1.8 |
- |
- |
- |
- |
0.08 |
In |
0.05 |
19% |
- |
- |
- |
0.07 |
- |
- |
- |
- |
- |
Sn |
0.5 |
4.8% |
4.3 |
3.3 |
0.52 |
- |
- |
- |
- |
- |
1.8 |
Sb |
0.05 |
6.0% |
- |
- |
0.09 |
0.15 |
- |
- |
- |
- |
- |
Te |
0.1 |
30% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Cs |
0.1 |
5.3% |
8.2 |
0.33 |
- |
0.39 |
- |
- |
- |
- |
- |
Ba |
0.1 |
4.8% |
38 |
1.4 |
5500 |
11 |
2.7 |
1.2 |
1.5 |
1.5 |
88 |
La |
0.05 |
2.1% |
- |
0.08 |
0.08 |
1.0 |
0.31 |
0.25 |
0.16 |
0.12 |
0.15 |
Ce |
0.05 |
3.1% |
0.06 |
0.16 |
0.06 |
1.7 |
0.24 |
0.20 |
0.11 |
0.09 |
0.29 |
Pr |
0.05 |
2.5% |
- |
- |
- |
0.28 |
- |
0.05 |
- |
- |
- |
Nd |
0.05 |
2.3% |
- |
0.1 |
- |
1.5 |
0.11 |
0.12 |
0.08 |
0.06 |
0.16 |
Sm |
0.05 |
11% |
- |
- |
- |
0.57 |
- |
- |
- |
- |
- |
Eu |
0.05 |
10% |
- |
- |
0.52 |
0.12 |
- |
- |
- |
- |
- |
Gd |
0.05 |
10% |
- |
- |
- |
0.91 |
- |
- |
- |
- |
- |
Tb |
0.05 |
3.6% |
- |
- |
- |
0.14 |
- |
- |
- |
- |
- |
Dy |
0.05 |
3.6% |
- |
- |
- |
1.0 |
- |
- |
- |
- |
- |
Ho |
0.05 |
5% |
- |
- |
- |
0.30 |
- |
- |
- |
- |
- |
Er |
0.05 |
4.9% |
- |
- |
- |
1.1 |
- |
- |
- |
- |
- |
Tm |
0.05 |
7.3% |
- |
- |
- |
0.20 |
- |
- |
- |
- |
- |
Yb |
0.05 |
7.1% |
- |
- |
- |
1.6 |
- |
- |
- |
- |
- |
Lu |
0.05 |
5.5% |
- |
- |
- |
0.33 |
- |
- |
- |
- |
- |
Hf |
0.05 |
8.7% |
0.08 |
0.25 |
- |
- |
- |
- |
- |
- |
- |
Ta |
0.05 |
16% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
W |
0.05 |
20% |
3.4 |
- |
- |
- |
- |
- |
- |
- |
- |
Os |
0.05 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Ir |
0.05 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Pt |
0.05 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Au |
0.05 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Hg |
0.5 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Tl |
0.05 |
11% |
0.28 |
- |
- |
0.05 |
- |
- |
- |
- |
- |
Pb |
0.05 |
4.1% |
3.1 |
0.43 |
0.76 |
280 |
3.8 |
2.9 |
0.48 |
0.75 |
4.0 |
Bi |
0.05 |
9.6% |
4.9 |
0.7 |
- |
0.11 |
- |
- |
0.14 |
0.1 |
0.32 |
Th |
0.05 |
5.5% |
- |
- |
- |
0.06 |
- |
- |
- |
- |
- |
U |
0.5 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
DL : Detection limit
%RSD presents the percentage relative standard deviation made as common estimate based on the relative standard deviation from duplicate determinations.
4.1.2 Analysis results – quantitative measurements
The analyses results from the quantitative measurements for inorganic substances in the selected kohl products is presented in the tables below.
It should be noticed that deviations may occur when comparing the quantitative results with the screening results. In that case the quantitative results are the preferred values as they consider circumstances
that may affect the analysis which is not the case to the same extent in the screening.
Table 2. Quantitative measurements of lead (Pb) in kohl products
Sample number |
Pb
mg/kg |
25 |
0.30 |
30 |
0.31 |
31 |
- |
33 |
- |
35 |
0.81 |
42 |
0.71 |
43 |
0.39 |
49 |
- |
51 |
0.86 |
52 |
2.8 |
56 |
0.36 |
59 |
0.78 |
60 |
280 |
61 |
4.2 |
62 |
3.1 |
64 |
0.53 |
65 |
0.84 |
66 |
4.2 |
%RSD |
6.6 |
”-” denotes measurement below detection limit of 0.1 mg/kg.
%RSD presents the percentage relative standard deviation made as common estimate based on the relative standard deviation from duplicate determinations.
Further quantitative determinations on elements were performed when a content higher than 10 mg/kg were observed in the screening with the exception of normally occurring elements such as aluminium,
calcium, iron, magnesium, silicon and titanium and the alkali metals. The results are listed in the table below.
Table 3. Quantitative measurements of other elements in kohl products
Sample number |
B
mg/kg |
Ba
mg/kg |
Bi
mg/kg |
Co
mg/kg |
Cu
mg/kg |
25 |
420 |
- |
- |
28 |
- |
30 |
- |
- |
- |
30 |
- |
31 |
- |
- |
- |
47 |
- |
33 |
- |
- |
- |
28 |
- |
35 |
- |
- |
- |
13 |
- |
42 |
- |
- |
- |
26 |
- |
43 |
- |
- |
- |
37 |
- |
49 |
- |
- |
- |
- |
- |
51 |
- |
- |
74900 |
- |
- |
52 |
- |
53 |
- |
33 |
- |
56 |
- |
- |
- |
25 |
- |
59 |
- |
6700 |
- |
- |
- |
60 |
32000 |
14,5 |
- |
- |
950 |
61 |
NA |
- |
- |
- |
- |
62 |
- |
- |
- |
- |
- |
64 |
- |
- |
- |
- |
- |
65 |
- |
- |
- |
- |
- |
66 |
NA |
NA |
- |
- |
- |
%RSD |
3.1 |
3.7 |
0.6 |
3.5 |
7.3 |
Table 3. continued
Sample number |
Mn
mg/kg |
Ni
mg/kg |
Sr
mg/kg |
V
mg/kg |
Zn
mg/kg |
25 |
465 |
31 |
- |
- |
- |
30 |
377 |
33 |
- |
- |
- |
31 |
710 |
23 |
- |
- |
- |
33 |
334 |
29 |
- |
- |
- |
35 |
270 |
37 |
- |
- |
- |
42 |
344 |
26 |
- |
- |
- |
43 |
421 |
41 |
- |
- |
- |
49 |
554 |
15 |
- |
- |
- |
51 |
490 |
- |
- |
- |
- |
52 |
474 |
38 |
- |
- |
- |
56 |
363 |
39 |
- |
- |
- |
59 |
297 |
17 |
120 |
- |
- |
60 |
190 |
65 |
280 |
74 |
115000 |
61 |
- |
55 |
- |
62 |
- |
62 |
- |
24 |
- |
- |
- |
64 |
- |
16 |
- |
- |
- |
65 |
- |
- |
- |
- |
- |
66 |
- |
- |
NA |
- |
- |
%RSD |
1.6 |
7.6 |
2.3 |
1.4 |
1.1 |
-: Below detection limit
NA: Not analysed
%RSD presents the percentage relative standard deviation made as common estimate based on the relative standard deviation from duplicate determinations.
It is noticed that:
- Lead (Pb) occurs in 15 out of 18 products above the detection limit of 0.1 mg/kg. The concentrations ranges 0.3 to 280 mg/kg. Lead occurs at the highest concentration in product no. 60 (280
mg/kg), cf. table 2.
- Boron (B) occurs in 2 products and at the highest concentration in product no. 60 (32000 mg/kg corresponding to 3.2%), cf. table 3.
- Barium (Ba) occurs in 3 products but only at high concentration in product no. 59 (6700 mg/kg).
- Bismuth (Bi) occurs in product no. 51 at high concentration (74900 mg/kg corresponding to 7.5%).
- Cobalt (Co) occurs in 9 out of 18 analysed products at the range 13 to 47 mg/kg.
- Manganese (Mn) occurs in 13 out of 18 products at the range 190 to 710 mg/kg.
- Nickel (Ni) occurs in 15 out of 18 products at the range 15 to 65 mg/kg.
- Strontium (Sr) occurs in 2 products at the range 120 to 280 mg/kg.
- Vanadium (V) occurs in 2 products at the range 62 to 74 mg/kg.
- Zinc (Zn) occurs in product no 60 at a high concentration (115000 mg/kg corresponding to 11.5% m/m).
4.2 Henna products
4.2.1 Analysis results – screening of inorganic substances
The inorganic analysis results from the screening of henna products are listed in the tables below.
Table 4. Henna products, inorganic analysis – screening analyses
Click here to see Table 4
Ana- lyte |
DL µg/g |
%RSD |
Sample no.: 31341- |
11 |
12 |
14 |
20 |
21 |
23 |
57 |
58 |
63 |
µg/g |
µg/g |
µg/g |
µg/g |
µg/g |
µg/g |
µg/g |
µg/g |
µg/g |
Li |
0.5 |
4.6% |
- |
2.9 |
- |
2.3 |
- |
5.9 |
1.5 |
1.4 |
1.6 |
Be |
1 |
14% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
B |
5 |
4.6% |
- |
31 |
- |
30 |
- |
63 |
42 |
25 |
22 |
Na |
1 |
1.9% |
3900 |
1200 |
> |
2900 |
4100 |
3600 |
3000 |
3600 |
1200 |
Mg |
1 |
1.7% |
1.3 |
4100 |
22 |
2700 |
1.3 |
5600 |
5500 |
8600 |
2600 |
Al |
1 |
4.7% |
4.5 |
1300 |
2.5 |
1400 |
7.8 |
3400 |
770 |
510 |
460 |
Si |
10 |
6.3% |
13 |
710 |
11 |
720 |
12 |
1000 |
380 |
330 |
580 |
K |
5 |
2.8% |
20 |
> |
74 |
5800 |
41 |
7300 |
2800 |
2600 |
5700 |
Ca |
5 |
2.8% |
13 |
15000 |
10 |
11000 |
6.2 |
16000 |
7500 |
6700 |
12000 |
Sc |
0.5 |
4.1% |
- |
- |
- |
0.53 |
- |
1.1 |
- |
- |
- |
Ti |
0.5 |
6.5% |
- |
57 |
- |
56 |
- |
160 |
27 |
29 |
29 |
V |
0.5 |
4.4% |
- |
2.9 |
- |
2.5 |
- |
8.7 |
2.3 |
2.0 |
1.5 |
Cr |
0.5 |
2.2% |
- |
3.3 |
- |
10 |
- |
6.0 |
3.2 |
5.7 |
5.7 |
Mn |
0.1 |
0.99% |
- |
78 |
- |
68 |
- |
170 |
35 |
46 |
95 |
Fe |
10 |
8.2% |
- |
1500 |
- |
1600 |
- |
2400 |
810 |
640 |
600 |
Co |
0.1 |
5.6% |
- |
0.90 |
- |
0.7 |
- |
2.0 |
0.59 |
0.62 |
0.78 |
Ni |
0.5 |
4.4% |
- |
3.9 |
- |
3.0 |
- |
8.7 |
2.1 |
1.9 |
1.9 |
Cu |
0.1 |
3.6% |
0.20 |
7.8 |
0.14 |
4.9 |
0.17 |
12 |
4.4 |
5.1 |
8.1 |
Zn |
0.5 |
6.8% |
1.4 |
20 |
19 |
11 |
3.0 |
22 |
76 |
71 |
14 |
Ga |
0.1 |
8.0% |
- |
0.45 |
- |
0.47 |
- |
0.97 |
0.30 |
0.21 |
0.22 |
As |
1 |
17% |
- |
- |
- |
- |
- |
2.3 |
- |
- |
- |
Se |
1 |
29% |
- |
- |
- |
- |
- |
- |
- |
- |
2.4 |
Rb |
0.1 |
2.9% |
- |
5.1 |
- |
9.6 |
- |
3.3 |
2.3 |
1.5 |
9.6 |
Sr |
0.1 |
2.1% |
- |
180 |
0.13 |
91 |
- |
210 |
180 |
210 |
120 |
Y |
0.1 |
2.4% |
- |
0.62 |
- |
0.80 |
- |
1.5 |
1.1 |
0.89 |
0.36 |
Zr |
0.1 |
11% |
- |
- |
- |
0.68 |
- |
0.60 |
0.20 |
- |
0.14 |
Nb |
0.1 |
3.1% |
- |
0.19 |
- |
0.26 |
- |
0.20 |
- |
- |
- |
Mo |
0.1 |
4.9% |
- |
1.7 |
- |
0.75 |
- |
0.60 |
0.17 |
0.15 |
0.29 |
Ru |
0.1 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Pd |
0.1 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Ag |
0.1 |
1.8% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Cd |
0.05 |
12% |
- |
0.06 |
- |
- |
- |
0.07 |
- |
- |
0.07 |
In |
0.05 |
19% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Sn |
0.5 |
4.8% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Sb |
0.05 |
6.0% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Te |
0.1 |
30% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Cs |
0.1 |
5.3% |
- |
0.30 |
- |
0.43 |
- |
0.46 |
0.28 |
0.21 |
0.19 |
Ba |
0.1 |
4.8% |
0.61 |
32 |
0.30 |
15 |
0.32 |
15 |
160000 |
140000 |
51 |
La |
0.05 |
2.1% |
- |
0.72 |
- |
1.6 |
- |
1.3 |
1.3 |
1.1 |
0.43 |
Ce |
0.05 |
3.1% |
- |
1.6 |
- |
3.2 |
- |
2.8 |
1.3 |
0.86 |
0.88 |
Pr |
0.05 |
2.5% |
- |
0.20 |
- |
0.38 |
- |
0.36 |
0.17 |
0.11 |
0.11 |
Nd |
0.05 |
2.3% |
- |
0.84 |
- |
1.5 |
- |
1.6 |
0.7 |
0.46 |
0.45 |
Sm |
0.05 |
11% |
- |
0.19 |
- |
0.31 |
- |
0.36 |
0.71 |
0.65 |
0.11 |
Eu |
0.05 |
10% |
- |
- |
- |
- |
- |
0.09 |
12 |
12 |
- |
Gd |
0.05 |
10% |
- |
0.21 |
- |
0.30 |
- |
0.42 |
0.17 |
0.12 |
0.11 |
Tb |
0.05 |
3.6% |
- |
- |
- |
- |
- |
0.06 |
- |
- |
- |
Dy |
0.05 |
3.6% |
- |
0.14 |
- |
0.19 |
- |
0.33 |
0.12 |
0.08 |
0.08 |
Ho |
0.05 |
5% |
- |
- |
- |
- |
- |
0.06 |
- |
- |
- |
Er |
0.05 |
4.9% |
- |
0.06 |
- |
0.09 |
- |
0.16 |
0.06 |
- |
- |
Tm |
0.05 |
7.3% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Yb |
0.05 |
7.1% |
- |
- |
- |
0.06 |
- |
0.14 |
0.05 |
- |
- |
Lu |
0.05 |
5.5% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Hf |
0.05 |
8.7% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Ta |
0.05 |
16% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
W |
0.05 |
20% |
- |
0.1 |
- |
0.17 |
- |
- |
- |
- |
- |
Os |
0.05 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Ir |
0.05 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Pt |
0.05 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Au |
0.05 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Hg |
0.5 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Tl |
0.05 |
11% |
- |
- |
- |
- |
- |
- |
- |
- |
- |
Pb |
0.05 |
4.1% |
- |
1.1 |
- |
1.3 |
- |
1.5 |
0.62 |
0.46 |
1.3 |
Bi |
0.05 |
9.6% |
- |
0.09 |
- |
- |
- |
- |
- |
- |
- |
Th |
0.05 |
5.5% |
- |
0.51 |
- |
1.8 |
- |
0.43 |
0.55 |
0.36 |
0.32 |
U |
0.5 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
DL: Detection limit
%RSD presents the percentage relative standard deviation made as common estimate based on the relative standard deviation from duplicate determinations.
4.2.2 Inorganic quantitative measurements
The quantitative measurements of inorganic substances in the selected henna products are shown in the tables below.
Table 5. Quantitative measurements of lead (Pb) in henna products
Sample number |
Pb
mg/kg |
1 |
- |
2 |
1.37 |
3 |
0.89 |
4 |
- |
5 |
2.0 |
8 |
1.5 |
9 |
- |
10 |
- |
11 |
- |
12 |
1.24 |
14 |
- |
20 |
1.5 |
21 |
- |
23 |
1.7 |
57 |
0.57 |
58 |
0.47 |
63 |
1.3 |
%RSD |
6.7 |
”-” denotes measurement below detection limit of 0.1 mg/kg.
%RSD presents the percentage relative standard deviation made as common estimate based on the relative standard deviation from duplicate determinations.
Correspondingly, quantitative determinations of elements, which at the screening were measured at a concentration above 10 mg/kg, were performed. However with the exception of normally occurring
elements such as aluminium, calcium, iron, magnesium, silicon and titanium and the alkali metals, which were included. The results are listed in the tables below.
Table 6. Quantitative measurements of other elements in henna products
Sample number |
B
mg/kg |
Ba
mg/kg |
Mn
mg/kg |
Sr
mg/kg |
1 |
- |
- |
- |
- |
2 |
112 |
37 |
189 |
407 |
3 |
157 |
47 |
230 |
710 |
4 |
- |
- |
- |
- |
5 |
110 |
31 |
144 |
406 |
8 |
48 |
24 |
78 |
219 |
9 |
- |
- |
- |
- |
10 |
- |
- |
- |
- |
11 |
- |
- |
- |
- |
12 |
60 |
49 |
104 |
272 |
14 |
- |
- |
- |
- |
20 |
57 |
26 |
91 |
145 |
21 |
- |
- |
- |
- |
23 |
111 |
24 |
234 |
307 |
57 |
53 |
178000 |
45 |
296 |
58 |
35 |
170000 |
65 |
357 |
63 |
43 |
65 |
122 |
180 |
%RSD |
3.0 |
6.7 |
2.2 |
2.1 |
”-” denotes measurement below detection limit of 0.1 mg/kg.
%RSD presents the percentage relative standard deviation made as common estimate based on the relative standard deviation from duplicate determinations.
It is noted that:
- Lead (Pb) occurs in 10 out of 17 products at the range 0.5 to 2.0 mg/kg.
- Boron (B) occurs in 10 out of 17 products at the range 35 to 157 mg/kg.
- Barium (Ba) occurs in 10 out of 17 products at the range 24 to 178000 mg/kg. Barium was found at high concentration in product no. 57 (178000 mg/kg corresponding to 17.8% m/m) and at almost
the same concentration in product no 58. The remaining products were found at the range 24 to 65 mg/kg.
- Manganese (Mn) occurs in 10 out of 17 products at the range 45 to 234 mg/kg.
- Strontium (Sr) occurs in 10 out of 17 products at the range 145 to 710 mg/kg.
4.2.3 Organic quantitative measurements
The primarily selected 17 henna products were analysed quantitatively for the content of para-phenylenediamine and lawsone.
p-Phenylenediamine was found in 3 products: In product no. 58 at a low concentration near the detection limit. In product no. 11 in a tube containing paste, p-phenylenediamine was found at 5000 mg/kg
corresponding to 0.5% m/m. In product no. 57, 170000 mg/kg was found, corresponding to 17% m/m, cf. table 7.
Based on the results of the lawsone analyses, the Danish Environment Protection Agency decided that also the remaining henna products should be analysed for lawsone.
The results are presented below.
Table 7. Quantitative measurements of p-phenylenediamine and Lawsone in henna products
Sample number |
p-phenylenediamine
mg/kg |
Lawsone
mg/kg |
1 |
< 20 |
< 20 |
2 |
< 2 |
310 |
3 |
< 2 |
40 |
4 |
< 2 |
< 20 |
5 |
< 2 |
3300 |
8 |
< 2 |
1300 |
9 |
< 2 |
< 20 |
10 |
< 2 |
< 20 |
11a (bag with paste) |
< 2 |
|
11b (tube with paste) |
5000 |
< 20 |
12 |
< 10 |
1100 |
14 |
< 2 |
390 |
20 |
< 20 |
160 |
21 |
< 2 |
< 20 |
23 |
< 20 |
1500 |
57 |
170000 |
50 |
58 |
27 |
190 |
63 |
< 20 |
3400 |
%RSD* |
11 |
26 |
Supplementary measurements for lawsone: |
4 |
|
< 20 |
6 |
|
2100 |
7 |
|
730 |
13 |
|
< 20 |
15 |
|
< 20 |
17 |
|
< 20 |
18 |
|
91 |
22 |
|
< 20 |
%RSD |
|
11 |
*: %RSD presents the percentage relative standard deviation made as common estimate based on the relative standard deviation from duplicate determinations.
Lawsone was found in 11 out of the 17 initially analysed products above the detection limit of 20 µg/g (= mg/kg). The 16 of the 17 samples had declared to contain lawsone (Lawsonia inermis). Only
product no. 3 had not declared to contain lawsone or Lawsonia inermis but a different plant species (Indigofera tinctoria).
The highest concentration of lawsone was found in product no. 63 that was a foreign import from India and without an ingredients list. The highest found concentration was 3400 mg/kg corresponding to
0.34% (m/m). The next highest concentration of lawsone was found in product no. 5 manufactured in the United Kingdom. The product contained 3300 mg/kg corresponding to 0.33% (m/m). The remaining
products contained lawsone below the detection limit (6 products) or had concentrations between 38 and 1500 mg/kg, i.e. between 0.004 and 0.15% of the product.
In the supplementary analyses, a further 8 products were analysed. Five of the products contained lawsone below the detection limit while 3 products were above the detection limit containing 91, 730 and
2100 mg/kg, respectively, cf. table 7. The concentration of lawsone in product no. 6 of 2100 mg/kg corresponds to 0.21% (m/m). Product no. 6 is produced by the same manufacturer in United Kingdom
as the aforementioned product no. 5.
| Front page | | Contents | | Previous | | Next | | Top |
Version 1.0 September 2005, © Danish Environmental Protection Agency
|