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Development of an analysis method to determine peroxides in or released from tooth-whitening/dental-bleaching products.

Contents

Foreword

Summary and conclusions

• Development of a catalase bioassay for the determination of peroxide
• Need for an improved method to determine active substances in tooth-whitening products
• Establishment of a protocol for the determination of peroxides using an enzymatic bioassay
• Main conclusions
• Project results - protocol to determine free or bound hydrogen peroxide in tooth-whitening agents

1 Introduction

2 Analysis of peroxides

• 2.1 Chemical analysis of peroxide in tooth-whitening products
• 2.2 Analysis of peroxide using catalase bioassay

3 Development and testing of catalase bioassay to determine peroxides

• 3.1 Technical design of measuring chamber, probe and data collection
  • 3.1.1 Test set-up
  • 3.1.2 Testing of the stability of the probe
  • 3.1.3 Testing of the tightness of the reaction chamber
• 3.2 Determination of the optimal conditions for the catalase bioassay
  • 3.2.1 Testing the optimal measurement scope and lower detection limit of the method
  • 3.2.2 Testing of the stability of the catalase
• 3.3 Testing of the catalase bioassay on peroxides in pure form
  • 3.3.1 Hydrogen and urea peroxide
  • 3.3.2 Calcium peroxide
  • 3.3.3 Sodium perborates and sodium percarbonate
  • 3.3.4 Overview of recovery percentages and accuracy for the different peroxides measured in the catalase bioassay

4 4 Tests on products

5 Assessment of results and conclusion

6 References

Anex 1: Technical specifications of the oxygen probe.

Annex 2: Measuring equipment used

Annex 3: Test of the catalase bioassay on tooth-whitening products, curves

Annex 4: Data from titrations and catalase bioassay

Annex 5: Draft Japanese Industrial Standard for tooth-whitening agents.

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Version 1.0 Marts 2009, © Danish Environmental Protection Agency