Evaluation of in vitro assays for determination of estrogenic activity in the environment

TABLE 1. EEQ levels found in different environmental matrices in various countries by use of in vitro assays Matrix EEQ (ng/l) Country In vitro assay Reference Wastewater influent 120 0.6-153 35-72 The Netherlands The Netherlands Japan ER-CALUX ER-CALUX YES Murk et al., 2002 Vethaak et al., 2002 Onda et al ., 2002 Wastewater effluent 2-25 0.2-7.8 6 16 0.05-2.6 <d.l-4.46 £1-15 1.90-14.9 21-147 4-35 Germany Germany Germany The Netherlands The Netherlands Belgium USA USA USA Japan E-screen E-screen E-screen ER-CALUX ER-CALUX YES YES MVLN YES YES Körner et al ., 1999 Körner et al ., 2001 Körner et al ., 2000 Murk et al., 2002 Vethaak et al., 2002 Witters et al., 2001 Huggett et al ., 2003 Snyder et al., 2001 Tilton et al., 2002 Onda et al ., 2002 Surface water 0.5 0.02-0.04 <d.l-81.4 <24 0.86-10.9 2.2-12.1 0.5-7.4 3.5 10 <d.l.-23.5 The Netherlands The Netherlands Belgium United Kingdom USA China Korea Japan Japan South Africa ER-CALUX ER-CALUX YES YES MVLN MVLN, HGELN E-screen E-screen YES YES Murk et al., 2002 Vethaak et al., 2002 Witters et al., 2001 Thomas et al., 2001 Snyder et al., 2001 Shen et al., 2001 Oh et al ., 2000 Behnish et al., 2001 Tashiro et al., 2003 Arijs et al., 2002 <d.l: below detection limit