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Evaluation of in vitro assays for determination of estrogenic activity in the environment
Table of Contents
Preface
1 Summary (English)
2 Resumé (dansk)
3 Description of the used methods and cell cultures
3.1 ER binding assays
3.2 Reporter gene assays
3.2.1 Mammalian-based reporter gene assays
3.2.2 Yeast-based reporter gene assay
3.3 Cell proliferation assays
3.3.1 E-screen assay
4 Estrogenic activity in aquatic environmental samples assessed by in vitro assays
5 Evaluation of the various methods
5.1 Sample preparation
5.1.1 Collection and storage
5.1.2 Filtration
5.1.3 Extraction
5.2 Laboratory facility requirements
5.3 Detection limits and EC50 values
5.3.1 Detection limits and EC50 values for the various in vitro assays
5.3.2 Detection limit and limit of quantification for the whole method
5.4 Time and cost considerations
5.5 Robustness
5.6 Utility in various matrices
5.7 Advantages and limitations compared to chemical analyses
5.8 Limitations compared to in vivo assays
6 Discussion and recommendations
7 Conclusions
8 Konklusioner
9 Reference list
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