| Front page | | Contents | | Previous | | Next |

Degradation of Estrogens in Sewage Treatment Processes

Appendix 1:
Instrumentation details

BOD₅: WTW Oxitop OC100/ Oxitop C100. Method according to WTW Application report No. BOD 266230.

Data fitting and statistics: GraphPad Prism version 3.00/4.00 for Windows, GraphPad Software, San Diego California USA, www.graphpad.com".

Nitrate: Merck Nitrate Test 0.2-25,0 mg/L NO₃--N Spectroquant® #114773. Quantified by adsorption at 517,1 nm as measured with a Specord S100 from Carl Zeiss Technology.

Oxygen: WTW Oxi323.

pH: Radiometer PHM95.

Steroid Estrogens analysis: HPLC separation was done with a C18 XTerra®RP₁₈ 3,5m3.0x150mm column and gradient elution by H2O:MeOH from 35:65 to 0:100. Ionisation by Atmospheric Pressure Photo Ionisation (APPI) with the Sciex API 3000 triplequad-MS-MS for detection.

| Front page | | Contents | | Previous | | Next | | Top |

Version 1.0 November 2004, © Danish Environmental Protection Agency