The Effects of Selected Pyrethroids on Embryos of Bombina bombina during different Culture and Semi-field Conditions

Appendix B

The composition of the culture media

FETAX solution water

625 mg NaCl
96 mg NaHCO3
30 mg Kcl
15 mg CaCl2
60 mg CaSO42H20
75 mg MgSO4

per litre of deionised or distilled water. The pH of the final solution should be 7.6 to 7.9. All chemicals were reagent-grade or better.

 



Version 1.0 September 2004, © Danish Environmental Protection Agency