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The Effects of Selected Pyrethroids on Embryos of Bombina bombina
during different Culture and Semi-field Conditions
Appendix C
The effect of esfenvalerate on malformation, growth inhibition, and mortality
Table C.1
The effect of esfenvalerate on malformation, growth inhibition, and mortality in Bombina bombina at 20°C in FETAX solution. Mean of 3 experiments.
Effeketen af esfenvalerat på Bombina bombina illustreret ved sublethale defekter, væksthæmning og dødelighed ved 20°C i FETAX opløsningen. Middeltal af 3 forsøg.
| Concentrations |
Malformation
( in procent) |
Growth inhibition
(in procent of controls) |
Mortality
(in procent) |
| Control |
1.8 (S.D. = 2.5) |
100.0 (S.D. = 0.0) |
3.3 (S.D. = 2.4) |
| 1.0 μg/l |
3.7 (S.D. = 5.2) |
97.9 (S.D. = 3.9) |
3.3 (S.D. = 4.7) |
| 2.5 μg/l |
0.0 (S.D. = 0.0) |
100.7 (S.D. = 3.3) |
3.3 (S.D. = 4.7) |
| 5.0 μg/l |
13.7 (S.D. = 4.5) |
98.8 (S.D. = 2.9) |
3.3 (S.D. = 4.7) |
| 10.0 μg/l |
32.4 (S.D. = 8.9) |
99.1 (S.D. = 3.2) |
6.7 (S.D. = 4.7) |
| 50.0 μg/l |
71.1 (S.D. = 11.0) |
96.9 (S.D. = 3,2) |
6.7 (S.D. = 4.7) |
| 100 μg/l |
90.0 (S.D. = 8.2) |
99.3 (S.D. = 1.7) |
3.3 (S.D. = 4.7) |
| 150 μg/l |
90.0 (S.D. = 8.2) |
98.1 (S.D. = 3.1) |
3.3 (S.D. = 4.7) |
| 300 μg/l |
100.0 (S.D. = 0.0) |
100.1 (S.D. = 1.9) |
6.7 (S.D. = 4.7) |
Table C.2
The effect of esfenvalerate on malformation, growth inhibition, and mortality in Bombina bombina at 20°C in FETAX solution. The results are expressed as NOEC, LOEC and
EC50/LC50 after 216 h of exposure.
Effekten af esfenvalerat på Bombina bombina illustreret ved sublethale defekter, væksthæmning og dødelighed ved 20°C i FETAX opløsningen. Resultaterne er udtrykt ved
NOEC, LOEC, EC50/LC50 efter 216 timers eksponering.
| |
Malformations |
| NOEC |
LOEC |
EC50 |
| Means of 3 experiments |
2.5 μg/l |
5.0 μg/l |
24.7 μg/l |
| Standard deviations |
0.0 |
0.0 |
8.7 |
| The 95% confidence limits |
- |
- |
14.8-34.6 |
| |
Growth inhibition |
| NOEC |
LOEC |
EC50 |
| Means of 3 experiments |
300 μg/l |
300 μg/l > |
300 μg/l > |
| Standard deviations |
0.0 |
0.0 |
0.0 |
| The 95% confidence limits |
- |
- |
- |
| |
Mortality |
| NOEC |
LOEC |
LC50 |
| Means of 3 experiments |
300 μg/l |
300 μg/l > |
300 μg/l > |
| Standard deviations |
0.0 |
0.0 |
0.0 |
| The 95% confidence limits |
- |
- |
- |
Table C.3
The effect of esfenvalerate on malformation, growth inhibition, and mortality in Bombina bombina at 24°C in FETAX solution. Mean of 3 experiments.
Effeketen af esfenvalerat på Bombina bombina illustreret ved sublethale defekter, væksthæmning og dødelighed ved 24°C i FETAX opløsningen. Middeltal af 3 forsøg.
| Concentrations |
Malformation
( in procent) |
Growth inhibition
(in procent of controls) |
Mortality
(in procent) |
| Control |
3.4 (S.D. = 2.4) |
100.0 (S.D. = 0.0) |
1.7 (S.D. = 2.4) |
| 1.0 μg/l |
3.7 (S.D. = 5.2) |
98.6 (S.D. = 4.1) |
6.7 (S.D. = 4.7) |
| 2.5 μg/l |
6.7 (S.D. = 4.7) |
97.6 (S.D. = 3.8) |
0.0 (S.D. = 0.0) |
| 5.0 μg/l |
14.7 (S.D. = 5.8) |
98.6 (S.D. = 2.0) |
6.7 (S.D. = 4.7) |
| 10.0 μg/l |
21.3 (S.D. = 7.8) |
96.5 (S.D. = 3.5) |
6.7 (S.D. = 4.7) |
| 50.0 μg/l |
61.8 (S.D. = 6.1) |
96.8 (S.D. = 3,0) |
3.3 (S.D. = 4.7) |
| 100 μg/l |
75.5 (S.D. = 6.3) |
95.5 (S.D. = 4.7) |
0.0 (S.D. = 0.0) |
| 150 μg/l |
96.6 (S.D. = 4.7) |
96.2 (S.D. = 4.2) |
6.7 (S.D. = 9.4) |
| 300 μg/l |
100.0 (S.D. = 0.0) |
98.6 (S.D. = 2.6) |
3.3 (S.D. = 4.7) |
Table C.4
The effect of esfenvalerate on malformation, growth inhibition, and mortality in Bombina bombina at 24°C in FETAX solution. The results are expressed as NOEC, LOEC and
EC50/LC50 after 120 h of exposure.
Effekten af esfenvalerat på Bombina bombina illustreret ved sublethale defekter, væksthæmning og dødelighed ved 24°C i FETAX opløsningen. Resultaterne er udtrykt ved NOEC,
LOEC, EC50/LC50 efter 120 timers eksponering.
| |
Malformations |
| NOEC |
LOEC |
EC50 |
| Means of 3 experiments |
2.5 μg/l |
5.0 μg/l |
32.7 μg/l |
| Standard deviations |
0.0 |
0.0 |
3.3 |
| The 95% confidence limits |
- |
- |
29.0-36.4 |
| |
Growth inhibition |
| NOEC |
LOEC |
EC50 |
| Means of 3 experiments |
300 μg/l |
300 μg/l > |
300 μg/l > |
| Standard deviations |
0.0 |
0.0 |
0.0 |
| |
- |
- |
- |
| |
Mortality |
| NOEC |
LOEC |
LC50 |
| Means of 3 experiments |
300 μg/l |
300 μg/l > |
300 μg/l > |
| Standard deviations |
0.0 |
0.0 |
0.0 |
| The 95% confidence limits |
- |
- |
- |
Table C.5
The effect of esfenvalerate in Bombina bombina on malformation, growth inhibition, and mortality at 20°C in Pond water. Mean of 3 experiments.
Effeketen af esfenvalerat på Bombina bombina illustreret ved sublethale defekter, væksthæmning og dødelighed ved 20°C i vandhulds vand. Middeltal af 3 forsøg.
| Concentrations |
Malformation
( in procent) |
Growth inhibition
(in procent of controls) |
Mortality
(in procent) |
| Control |
3.4 (S.D. = 2.4) |
100.0 (S.D. = 0.0) |
1.7 (S.D. = 2.4) |
| 1.0 μg/l |
3.7 (S.D. = 5.2) |
102.6 (S.D. = 3.0) |
3.3 (S.D. = 4.7) |
| 2.5 μg/l |
3.7 (S.D. = 5.2) |
100.8 (S.D. = 3.5) |
3.3 (S.D. = 4.7) |
| 5.0 μg/l |
6.7 (S.D. = 4.7) |
100.1 (S.D. = 4.9) |
3.3 (S.D. = 4.7) |
| 10.0 μg/l |
14.3 (S.D. = 6.1) |
100.8 (S.D. = 3.2) |
3.3 (S.D. = 4.7) |
| 50.0 μg/l |
54.0 (S.D. = 10.9) |
98.9 (S.D. = 1,6) |
3.3 (S.D. = 4.7) |
| 100 μg/l |
72.2 (S.D. = 5.7) |
99.0 (S.D. = 3.8) |
3.3 (S.D. = 4.7) |
| 150 μg/l |
90.0 (S.D. = 8.2) |
99.8 (S.D. = 4.3) |
3.3 (S.D. = 4.7) |
| 300 μg/l |
96.7 (S.D. = 4.7) |
98.3 (S.D. = 5.4) |
3.3 (S.D. = 4.7) |
Table C.6
The effect of esfenvalerate on malformation, growth inhibition, and mortality in Bombina bombina at 20°C in pond water. The results are expressed as NOEC, LOEC and EC50/LC50
after 216 h of exposure.
Effekten af esfenvalerat på Bombina bombina illustreret ved sublethale defekter, væksthæmning og dødelighed ved 20°C i vandhuldsvand. Resultaterne er udtrykt ved
NOEC, LOEC, EC50/LC50 efter 216 timers eksponering.
| |
Malformations |
| NOEC |
LOEC |
EC50 |
| Means of 3 experiments |
5.0 μg/l |
10.0 μg/l |
43.7 μg/l |
| Standard deviations |
0.0 |
0.0 |
9.0 |
| The 95% confidence limits |
- |
- |
40.0-47.4 |
| |
Growth inhibition |
| NOEC |
LOEC |
EC50 |
| Means of 3 experiments |
300 μg/l |
300 μg/l > |
300 μg/l > |
| Standard deviations |
0.0 |
0.0 |
0.0 |
| The 95% confidence limits |
- |
- |
- |
| |
Mortality |
| NOEC |
LOEC |
LC50 |
| Means of 3 experiments |
300 μg/l |
300 μg/l > |
300 μg/l > |
| Standard deviations |
0.0 |
0.0 |
0.0 |
| The 95% confidence limits |
- |
- |
- |
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Version 1.0 September 2004, © Danish Environmental Protection Agency
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