Effects of Pesticides on Bombina bombina in Natural Pond Ecosystems

Appendix A

Table A.1:
Pesticides used on field(s) surrounding the ponds. Inaccurate dates are marked with (*). Where two fields surrounded one pond, the fields are indicated by roman numbers.

Brug af pesticider på mark(er) omkring vandhullerne. Upræcise datoer er markeret med (*). Hvor to marker omgav et vandhul er markerne angivet med romertal.

Pond Year Crop Spraying
date
Pesticide commercial name and dose Pesticide active compound
P1 1993 Winter rape (I)
Wheat (II)
15.4.93* (II) Ally 20 DF 15 g/ha
DLG M propionat 50 1.5 l/ha
Metsulfurone-methyl
Mechlorprop-P
  1994 Fallow (I)
Barley (II)
20.4.94 (II) Tilt Top 0.2 l/ha
Express 11.25 g/ha
Propiconazol; Fenpropimorph
Tribenurone
      30.5.94 (II) Tilt Top 0.2 l/ha
Pirimor 0.2 kg/ha
Propiconazol; Fenpropimorph
Pirimicarb
  1995 Winter wheat 2.5.95 Express 7.5 g/ha
Starane 180 0.1 l/ha
Tribenurone
Fluroxypyr
      25.6.95 Tilt Top 0.25 l/ha
Pirimor 0.15 kg/ha
Propiconazol; Fenpropimorph
Pirimicarb
      9.7.95 Pirimor 0.25 kg/ha Pirimicarb
      20.9.95 Roundup 1.25 l/ha Glyphosat
P2 1993 Barley 4.5.93 (I+II) Shellprox Super F 3.25 l/ha 2,4D; MCPA; Dichlorprop
    (I+II) 14.5.93 (I+II) Shellprox Super F 3.25 l/ha 2,4D; MCPA; Dichlorprop
      2.6.93 (I+II) Avenge 3 l/ha Difenzoquat-methylsulfat
      28.10.93 (II) Mylone Power 0.6 l/ha
Arelon 1.0 l/ha
Ioxynil; Mechlorprop
Isoproturon
  1994 Barley (I) 11.5.94 (II) Tilt Top 0.3 l/ha Propiconazol; Fenpropimorph
    Wheat (II) 12.5.94 (I) Tilt Top 0.3 l/ha Propiconazol; Fenpropimorph
      1.6.94* (I+II) Arelon 2.94 l/ha Isoproturon
      3.6.94 (II) Tilt Top 0.3 l/ha
Pirimor 0.15 kg/ha
Propiconazol; Fenpropimorph
Pirimicarb
      4.6.94 (I) Tilt Top 0.3 l/ha
Sumicidin 0.2 l/ha
Propiconazol; Fenpropimorph
Fenvalerate
      20.6.94 (II) Tilt Top 0.3 l/ha
Sumicidin 0.2 l/ha
Propiconazol; Fenpropimorph
Fenvalerate
      Aut. 94* (I+II) Stomp SC 0.7 l/ha
Arelon fl. E 0.7 l/ha
Pendimethaline
Isoproturon
  1995 Winter barley 14.5.95 Tilt Top 0.3 l/ha Propiconazol; Fenpropimorph
P3 1993 Wheat 15.4.93* Ally 20 DF 15 g/ha
DLG M propionat 50 1.5 l/ha
Metsulfuron-methyl
Mechlorprop
      12.6.93 Tilt Top 0.2 l/ha
Pirimor 0.25 kg/ha
Propiconazol; Fenpropimorph
Pirimicarb
  1994 Fallow      
  1995 Winter wheat 16.5.95 Express 7.5 g/ha
Starane 180 0.1 l/ha
Tribenurone
Fluroxypyr
      25.6.95 Tilt Top 0.25 l/ha
Pirimor 0.15 kg/ha
Propiconazol; Fenpropimorph
Pirimicarb
      9.7.95 Pirimor 0.25 kg/ha Pirimicarb
P4 1993 Wheat 15.4.93* Ally 20 DF 15 g/ha
DLG M propionat 50 1.5 l/ha
Metsulfuron-methyl
Mechlorprop
      12.6.93 Tilt Top 0.2 l/ha
Pirimor 0.25 kg/ha
Propiconazol; Fenpropimorph
Pirimicarb
  1994 Fallow      
Pond Year Crop Spraying
date
Pesticide commercial name and dose Pesticide active compound
P4 1995 Wheat 16.5.95 Express 7.5 g/ha
Starane 180 0.1 l/ha
Tribenurone
Fluroxypyr
      25.6.95 Tilt Top 0.25 l/ha
Pirimor 0.15 kg/ha
Propiconazol; Fenpropimorph
Pirimicarb
      9.7.95 Pirimor 0.25 kg/ha Pirimicarb
P5 1993 Beet (I)
Barley (II)
5.4.93* (II) Express 75 DF 7.5 g/ha
Starane Mixer 0.3 l/ha
Tribenurone
Fluroxypyr
      5.5.93* (I) Goltix 2 kg/ha
Betaron 2 l/ha
Metamitrone
Phenmedipham; Ethofumesat
      15.5.93* (I) Goltix 2 kg/ha
Betaron 2 l/ha
Metamitrone
Phenmedipham; Ethofumesat
  1994 Beet (I)
Barley (II)
20.4.94 (II) Tilt top 0,2 l/ha
Express 11,25 g/ha
Propiconazol; Fenpropimorph
Tribenurone
      20.5.94 (I) Goltix WG 1 kg/ha
Betanal progress 1 kg/ha
Metamitrone
Phenmedipham; Ethofumesat; Desmedipham
      29.5.94 (I) Goltix WG 1.0 kg/ha
Betanal progress 1.0 l/ha
Metamitron
Phenmedipham; Ethofumesat; Desmedipham
      30.5.94 (II) Tilt Top 0.2 l/ha
Pirimor 0.2 kg/ha
Propiconazol; Fenpropimorph
Pirimicarb
      10.6.94 (I) Pirimor 0.3 kg/ha Pirimicarb
  1995 Beet (I)
Barley (II)
10.5.95* (I) Betanal OF 1 l/ha
Goltix WG 1 l/ha
Phenmedipham
Metamitron
      15.5.95* (II) Express (slop)
Tilt top 0.3 l/ha
Starane 180 (slop)
Harmony (slop)
Tribenurone
Propiconazol; Fenpropimorph
Fluroxypyr
Thifensulfurone
      20.5.95* (I) Betanal OF 1 l/ha
Goltix WG 1 l/ha
Phenmedipham
Metamitron
      5.6.95* (II) Tilt top 0.3 l/ha Propiconazol, Fenpropimorph
P6 1993 Winter rape (I)
Winter barley (II)
Aut. 1992-May 1993* (I) Sumi Alpha 5 FW 0.2 l/ha
Matrigon 1.0 l/ha
Esfenvalerate
Clopyralide
      Aut. 1992-May 1993* (II) Tolkan 1.0 l/ha
Mylone Power 0.6 l/ha
Cerone 0.2 l/ha
Corbel 0.2 l/ha
Tilt Top 0.3 l/ha
Isoproturon
Ioxynil; Mechlorprop
Ethephone
Fenpropimorph
Fenpropimorph; Propiconazol
      1.11.93* (I) Tolkan 1.0 l/ha
Mylone Power 0.6 l/ha
Isoproturon
Ioxynil; Mechlorprop
  1994 Wheat (I) 11-16.5.94* (I) Tilt Top 0.3 l/ha Propiconazol; Fenpropimorph
    Rape (II) 3-10.6.94* (I) Tilt Top 0.3 l/ha
Pirimor 0.25 kg/ha
Propiconazol; Fenpropimorph
Pirimicarb
      20-27.6.94* (I) Tilt Top 0.3 l/ha Propiconazol; Fenpropimorph
      15.10.94 (I) Tolkan 1.0 l/ha
Mylone Power 0.6 l/ha
Isoproturon
Ioxynil; Mechlorprop
Pond Year Crop Spraying
date
Pesticide commercial name and dose Pesticide active compound
P6 1995 Winter barley (I)
Wheat (II)
11.5.95 (I) Tilt Top 0.3 l/ha
Terpal 1.0 l/ha
Propiconazol; Fenpropimorph
Ethephone; Mepiquat
      14.5.95 (II) Ally 20 DF 20 g/ha
Duplosan MP/D Kombi 1.5 l/ha
Cycocel 750 1.0 l/ha
Tilt Top 0.3 l/ha
Starane 180 0.4 l/ha
Metsulfurone
Mechlorprop; Mechlorprop-P
Chlormequat
Propiconazol; Fenpropimorph
Fluroxypyr
      26.6.95 (II) Tilt Top 0.3 l/ha
Pirimor 0.2 kg/ha
Propiconazol; Fenpropimorph
Pirimicarb
P7 1993 Fallow      
  1994 Wheat 13.5.94 Express 15 g/ha Tribenurone
      25.9.94 Touchdown 2.0 l/ha Glyphosat-trimesium
  1995 Barley (I) 26.5.95 (II) Spar 2 0.6 l/ha Phenmedipham; Ethofumesat
    Beet (II) 5.6.95 (I) Express 7.5 g/ha Tribenurone
      12.6.95 (II) Spar 2 0.75 l/ha Phenmedipham; Ethofumesat
P8 1993 Barley 29.4.93 Glean 20 DF 15 g/ha Chlorsulfurone
      26.5.93 Tilt-Megaturbo 0.2 l/ha
Sumi Alpha 0.2 l/ha
Dimethoat 0.2 l/ha
Propiconazol; Fenpropimorph
Esfenvalerate
Dimethoate
  1994 Winter barley 27.4.94 Tilt-Megaturbo 0.3 l/ha
Mylone Power 1.0 l/ha
Express 7.5 g/ha
Propiconazol; Fenpropimorph
Ioxynil; Mechlorprop
Tribenurone
      21.5.94 Tilt-Megaturbo 0.3 l/ha
Dimethoat 0.6 l/ha
Propiconazol; Fenpropimorph
Dimethoate
      20.6.94 Tilt-Megaturbo 0.5 l/ha
Dimethoat 0.2 l/ha
Sumi Alpha 0.2 l/ha
Propiconazol; Fenpropimorph
Dimethoate
Esfenvalerate
      25.8.94 Fusilade 0.3 l/ha Fluazifop-P-butyl
      8.10.94 Lontranil 1.0 l/ha Clopyralide; Cyanazine
      20.10.94 Kerb F 0.5 l/ha Propyzamide
  1995 Winter rape 20.4.95 Sumi Alpha 0.2 l/ha Esfenvalerate
      17.5.95 Derosal 0.7 l/ha Carbendazim
P9 1993 No inv.      
  1994 Fallow      
  1995 Wheat 16.5.95 Express 7.5 g/ha
Starane 180 0.1 l/ha
Tribenurone
Fluroxypyr
      25.6.95 Tilt Top 0.25 l/ha
Pirimor 0.15 kg/ha
Propiconazol; Fenpropimorph
Pirimicarb
      9.7.95 Pirimor 0.25 kg/ha Pirimicarb

Table A.2
Supply of fertilizer for the growth season on fields surrounding ponds. Unknown amount of fertilizer supplied are indicated by (?). Where two fields surround one pond, the fields are indicated by roman numbers.

Tilførsel af gødning til marker omkring vandhullerne. Ukendt gødningsmængde er markeret med (?). Hvor to marker omgiver et vandhul, er markerne angivet med romer tal.

Pond 1993 1994 1995
Fertilizer (kg/ha) Fertilizer (kg/ha) Fertilizer (kg/ha)
N P N P N P
P1 230 (I)
? (II)
26 (I)
? (II)
0 (I)
100 (II)
0 (I)
8 (II)
195 23
P2 0 0 128 (I)
115 (II)
0 (I)
0 (II)
? ?
P3 190 26 0 0 195 23
P4 190 26 0 0 195 23
P5 manure

(I+II)

manure

(I+II)

manure

(I+II)

manure

(I+II)

manure

(I+II)

Manure

(I+II)

P6 80 (I)
90 (II)
0 (I)
0 (II)
160 (I)
206 (II)
22.5 (I)
40.5 (II)
75 (I)
200 (II)
? (I+II)
P7 0 0 170 ? 84 (I)
84 (II)
12 (I)
12 (II)
P8 103 10 135 0 ? ?
P9     0 0 195 23

Grey cells indicates that the pond was not included in the investigation in 1993.

Table A.3
Pesticide concentrations in ponds (ng/l).

Pesticidkoncentrationer i vandhuller (ng/l)

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Table A.4:
Vegetation cover of ponds (%).

Vegetationsdække i vandhuller (%).

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Table A.5
Yearly survival of Bombina bombina older than 1 year in Danish localities and degree of cultivation of the habitat. Ponds, moors, dry and wet permanent grasslands, hedgerows and woods are uncultivated areas. Agricultural areas are cultivated. Survival estimates were calculated over a number of years.

Årlig overlevelse af klokkefrøer ældre end 1 år på danske lokaliteter og dyrkningsgrad af habitaten. Vandhuller, moser, enge, overdrev, hegn og skov er udyrkede arealer, mens landbrugsarealer er dyrkede arealer. Overlevelsesestimater er udregnet som gennemsnit over flere år.

Locality Cultivation
(%)
Period Average yearly
survival (%)
Agersø 80 1990-94 66
    1990-95 70
    1990-96 62
    1994-96 74
Avernakø 90 1990-91 54
    1993-95 56
    1994-95 60
Hjortø 51 1983-90 85
    1988-90 93
    1993-95 80
Knudshoved Odde east 0 1988-91 94
    1991-94 43
Knudshoved Odde west 0 1988-91 81
    1991-94 68
Nekselø 40 1989-93 73
    1990-94 77
    1993-94 75
    1993-96 73

Table A.6
Predators in enclosures during hatching experiment. Number of predators indicated by: - none, + 1, ++ 2-5.

Prædatorer i bure under klækningseksperiment. Antal prædatorer er markeret ved: - ingen, + 1, ++ 2-5.

  Predator P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11
1993 Dytiscidae     - - - - - -   -  
  Odonata     - - - - - -   -  
  Hirudinea     - - - - ++ -   -  
1994 Dytiscidae - - - - - - + - + - -
  Odonata - - - - - - - - - - -
  Hirudinea - - - - - - ++* - - - -
1995 Dytiscidae - - - - - - - - - - -
  Odonata - - - - - - - - - - -
  Hirudinea + - - - - - + - - - -

*A leach was observed swallowing a small tadpole.
In 1993-94 eggs were kept in bags with a mesh size of 1x1 mm. Leaches (Hirudinea) were able to get into these. In 1995 fine-meshed nylon bags were used instead. In P1 and P7 in 1995 a duplicate portion of eggs were kept in bags with mesh size 1x1 mm.

Grey cells indicate that the pond was not included in the investigation in 1993.

Table A.7
Predators in enclosures during tadpole growth experiment. Number of Dystiscidae and Odonata indicated by: - none, + 1, ++ 2-10. Number of Hirudinea indicated by: - none, + 1-10, ++ 11-20, +++ 21-50, ++++ >50.

Prædatorer i bure under haletudse eksperiment. Antal Dystiscidae og Odonata er markeret ved: - ingen, + 1, ++ 2-10. Antal Hirudinea er markeret ved: - ingen, + 1-10, ++ 11-20, +++ 21-50, ++++ >50.

  Predator P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11
1993 Dytiscidae - - + - - ++ - -   -  
  Odonata - - + - - - - ++   -  
  Hirudinea ++++ ++++ ++++ - - - +++ ++++   -  
1994 Dytiscidae - - - - - +++ - + - - +
  Odonata - - - - - - - - - - -
  Hirudinea ++++ - +++ - - ++++ ++++ +++ - - -
1995 Dytiscidae - - + - - - - - - - -
  Odonata - - - - - - - - - - -
  Hirudinea +++ ++ + ++ ++ +++ ++++ - + - -

Grey cells indicate that the pond was not included in the investigation in 1993.

Table A.8
Epiphytic coverings of algae, bacteria and protozoa in ponds in 1994..

Epifytiske belægninger af alger, bakterier og protozoer i vandhuller i 1994.

    P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11
Algae Achnantes           x          
  Anabaena x x x           x    
  Blue-green alga sp.                   x  
  Bulbochaete                 x    
  Chlorococcum             x   x    
  Chrysophyta x x x x x x x   x    
  Closterium   x       x x   x    
  Coeleastrum   x                  
  Coleochaete scutata x x x x   x     x    
  Coleosparum             x        
  Cosmarium             x        
  Euglenids   x                  
  Fragillaria       x         x    
  Green alga sp.               x     x
  Microcystes plac x                    
  Navicula     x       x        
  Nitzschia             x        
  Oedogonium x     x     x     x  
  Oscillatoria         x   x        
  Pediastrum                 x    
  Spirogyra x           x        
  Stigeoclonium   x     x x          
No. of algae species 6 7 4 4 3 4 10 1 8 3 1
Thickness of epiphytic algae* ++ +++ + ++ ++ +++ +++ + +++ + +
Bacteria Leptothrix** 1 2 0 0 0 1 0 0 2 0 0
  Sulphur bacteria         x x     x    
Protozoa Paramaecium   x   x   x          
  Amoeba                      
  Rotifera x   x   x       x    
  Vorticella         x       x    
Thickness of Protozoa*** 0 1 0 2 0 1 0   2    
Score for Food Quality Index 5 8 2 5 3 7 6 1 9 1 1
  P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11
                  May      
Algae Anabaena x x x x         x   x
  Cladophora           x          
  Clamydomonas     x           x    
  Closterium   x       x x        
  Chactophora       x   x       x x
  Chlorococcum           x       x x
  Chocleophora           x          
  Chrysophyta x x x x x   x x x x x
  Cocconeis x x x                
  Coleochaete x   x       x     x x
  Cosmarium           x         x
  Euglena sp.       x x x          
  Fragillaria                      
  Green algae sp.               x      
  Lyngbya     x   x            
  Mougeotia   x x x         x x  
  Navicula   x     x x x     x x
  Nitzschia   x x     x     x    
  Oedegonium   x   x           x x
  Oscillatoria   x     x x         x
  Dinophyceae                 x x  
  Pinapinaulam viridis   x                  
  Spirogyra   x   x   x     x x x
  Stigeoclonium x   x       x   x    
  Tribonema   x       x         x
  Volvox   x             x    
  Zygnema                     x
No. of algae species 5 13 9 7 5 12 5 2 9 9 12
Thickness of epiphytic algae* + ++ + +++ + +++ + + +++ + ++
Bacteria Leptothrix** 1 2 2 1 2 1 1 0 2 1 0
  Sulphur bacteria   x     x x   x x    
Protozoa Paramaecium x x x x x x x   x   x
  Vorticella       x              
  Amoeba       x   x          
  Rotifera       x   x x   x   x
Thickness of Protozoa*** 0 0 0 2 0 1 1 0 1 0 0
Score for Food Quality Index 4 9 7 7 3 8 5 1 8 5 6

*
+ = thin
++ = thick
+++ = very thick

**
0 = none
1 = few
2 = many

***
0 = none or very few
1 = few
2 = many

Grey cells indicate uncompleted registration of algae.

Table A.9
Water temperatures (depth 20 cm). Average temperatures are based on data in grey cells.

Vandtemperaturer i 20 cm's dybde. Gennemsnitsværdier er baseret på data i grå felter.

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Version 1.0 October 2004, © Danish Environmental Protection Agency