| Front page | | Contents | | Previous | | Next |

Effects of Pesticides on Bombina bombina in Natural Pond Ecosystems

Preface

This report is a part of the research programme "Effects of Pesticides on Ponds".

The projects were funded by the Danish Environmental Protection Agency' s Research programme on Environmental effects of pesticides.

The aim of the project was:

To develop a model-based tool for evaluation of risk related to pesticide exposure in surface water. The tool must be directly applicable by the Danish Environmental Protection Agency (DEPA) in their approval procedure. As part of this goal, the project had to:

1. Determine the direct and indirect effects of pesticides on ponds,
2. Validate E(L)C50 values from well-known algae – amphibian laboratory tests,
3. Develop an OECD test guideline for amphibians as proposed by Denmark in 1992,
4. To develop or fit a model for calculating starting concentrations (PIEC) and chronic concentrations (PEC) of pesticides in ponds for use in risk assessment.

The project consisted of four subprojects with individual objectives. The sub-projects are listed in Table 1.

Table 1. Sub-projects of " Effects of Pesticides on Ponds".

Tabel i. Oversigt over delprojekter i "Effekter af pesticider i vandhuller".

The reports produced by the projects are:

• Pedersen, C. L. (1999): Effects of the pesticides Esfenvalerate and Prochloraz on Pond Ecology. - Ministry of Environment, Danish Environmental Protection Agency, Pesticides Research No. 50.
• Larsen, J., & Sørensen, I. (2004): The effect of esfenvalerate and prochloraz on amphibians with reference to Xenopus laevis and Bombina bombina - Ministry of Environment, Danish Environmental Protection Agency, Pesticides Research No. 83.
• Larsen, J., Sørensen, I. & Gustavson, K. (2004): The effects of selected pyrethroids on embryos of Bombina bombina during different culture and semi-field conditions - Ministry of Environment, Danish Environmental Protection Agency, Pesticides Research No. 84.
• Briggs, L. & Damm, N. (2004): Effects of pesticides on Bombina bombina in natural pond ecosystems. - Ministry of Environment, Danish Environmental Protection Agency, Pesticides Research No. 85.
• Mogensen, B.B., Sørensen, P.B., Stuer-Lauridsen, F. & Lassen, P. (2004): Fate of pyrethroids in farmland ponds - Ministry of Environment, Danish Environmental Protection Agency, Pesticides Research No. 86.

The project was overseen by a steering committee. The members have made valuable contributions to the project. The committee consisted of:

Jens Mossin Danish Environmental Protection Agency
Lars Briggs Amphi concult
Leif Bisschop Larsen Funen County
Peter Wiberg-Larsen Funen County
Jørgen Larsen Danish Technological Institute
Betty Bügel Mogensen National Environmental Research Institute
rank Stuer-Lauridsen National Environmental Research Institute
Arne Schiøtz Danmarks Akvarium
Per Rosenkilde University of Copenhagen
Henning Clausen Danish Environmental Protection Agency
Claus Hansen Danish Environmental Protection Agency

For this particular report the following persons are thanked.

Lars Briggs and Anne Margrethe Andersen planned the investigation and conducted the field work and laboratory analysis. Anne Margrethe Andersen has thereby contributed significantly to the success of this investigation by taking a vital part of both the planning and the practical work. Anne Margrethe Andersen also participated in the analysis of the data and contributed with valuable discussions on the data.

John Frisenvænge has carried out the statistical calculations with great skill.

Erich Wederkinch, County of West Zealand and Ditte Guldager Christiansen kindly allowed the use of survival data on Bombina bombina.

Helge and Agnes Andersen kindly allowed the investigators to use their house on Avernakø during periods of field work. We will always remember their hospitality.

On the east coast of Fyn Svend Micleit and Ulrich Hansen of Taarup Strand Camping, Klintholm I/S and Harald Madsen of Stenagergaard kindly allowed entrance to ponds on their property, and are thanked for a good and constructive co-operation.

The following farmers kindly allowed the investigators access to ponds on their property and gave thorough reports of use of fertilizers and pesticides: on the east coast of Fyn: Jørgen Sømark, manager of Juelsberg Estate; on Avernakø: Allan Carlsen, Axel Klingenberg, Ole Klingenberg, Helge Kristensen, Erna Vindeløv. The landowners are thanked for a valuable and positive co-operation in the process of collecting data.

Jens Mossin, Per Rosenkilde, Peter Wiberg-Larsen, Leif Bisschop-Larsen, Henning Clausen and Claus Hansen gave valuable comments on the manuscript and constructive discussions. Per Rosenkilde has also helped by proofreading the report.

Mariusz Rybacki of the Polish Academy of Science, Research Centre for Agriculture and Forest Environment in Poznan has made drawings of the ponds and surroundings on computer and is thanked very much for this work.

Tyge Christensen, University of Copenhagen, Botanical Institute, department of Mycology and Phycology is thanked for assisting in the identification of algae to the level of genus and species. Poul Møller Pedersen, University of Copenhagen, Botanical Institute, department of Mycology and Phycology is thanked for orthographic corrections of names of species and genera of algae, bacteria and protozoa.

Members of the Nordic Herpetological Society have helped greatly by catching and photographing Bombina bombina (identification for calculation of survival).

The Danish Environmental Protection Agency is acknowledged for funding the project.

| Front page | | Contents | | Previous | | Next | | Top |

Version 1.0 October 2004, © Danish Environmental Protection Agency