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Survey of azo-colorants in Denmark

 

 

Appendix 3 Effect Concentration of the Metabolites.
CI-name CAS-no. Toxicity Effect

LC50, ppm

Ref.
    Organism   Fish Daph-
nia
Alga Bird Crus-
tacea
Mol-
lusc
 
Aniline                     
4-chloro- aniline 106-47-8 Wild Bird LD50-       100 mg/kg     ECDIN
    Oryzias latipes, juv LD50-48h 28           ECDIN
    Poecilia reticulata, juv LC50-14d 25           ECDIN
    Pimephales promelas LC50-96h 12           Dump. 87
    Salmo gairdneri LC50-96h 14           Dump. 87
    Lepomis macrochirus LC50-96h 2           Dump. 87
    Brachydanio rerio LC50-48h 46           Dump. 87
    Daphnia magna EC50-24h   0,06         Dump. 87
4 amino-
benzene
60-09-3 Oryzias latipes, juv LC50-24h 1,7           ECDIN
    Orizias latipes, juv LC50-48h 0,7           ECDIN
o-anisidine 90-04-0 Poecilia reticulata EC50-14d 18           R.A.1
    Poecilia reticulata LC50-14d 165           R.A.1
    Daphnia magna EC50-48h   6,8         R.A.1
    Daphnia magna LC50-48h   12         R.A.1
    Scenedesmus pannonicus EC50-96h     12       R.A.1
Toluidine                    
o-toluidine 95-53-4 Poecilia reticulata, juv LC50-14d 81            ECDIN
    Crassostrea gigas LD50-96h           100 ECDIN
    Mytilus edulis LD50-96h           100 ECDIN
    Daphnia magna LC100-24h   60         ECDIN
    Daphnia magna LC0-24h   2,5         ECDIN
    Daphnia magna LC50-24h   26         ECDIN
    Tetrahymena pyriformis EC50-24h     520       ECDIN
    Elasmopodus pectenicrus LC50-96h         >40   ECDIN
4-methyl-m-
phenyl-
enediamine
95-80-7 Chactogam-marus marinus LC50-96h          77   ECDIN
Benzidine                    
benzidine 92-87-5 Teredo diengensis, larvae LC100-72h           100 ECDIN
    Limnoria lignorum, adult LC100-18h         >0,05   ECDIN
    Limnoria tripunctata, adult LC50-100h          50   ECDIN
    Oryzias latipes, juv LC50-24h 16            ECDIN
    Oryzias latipes, juv LC50-48h 10,5             ECDIN

1 R.A. = Risk assessment. Federal Ministry of Environment,
Youth and Family and the Federal Chancellery (1997).