Environmental Project No. 1274 2009

Development of an analysis method to determine peroxides in or released from tooth-whitening/dental-bleaching products.

This project developed a catalase bioassay for the determination of the content of peroxide in tooth-whitening products. The catalase bioassay converts peroxide into oxygen, which is then measured with a probe. The catalase bioassay was tested on pure peroxides (hydrogen peroxide, urea peroxide, calcium peroxide, sodium perborate and sodium percarbonate), all of which are active substances in tooth-whitening products, as well as on a number of different tooth-whitening products on the Danish market. The newly developed catalase bioassay was moreover compared to chemical analysis methods. It was concluded that the catalase bioassay has some advantages over the chemical methods.

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Version 1.0 Marts 2009, © Danish Environmental Protection Agency