Development of an analysis method to determine peroxides in or released from tooth-whitening/dental-bleaching products.
Annex 3: Test of the catalase bioassay on tooth-whitening products, curves
This Annex shows the curves for seven tooth-whitening products, analysed with the newly developed catalase bioassay. All the products were analysed as described in the protocol, and a known quantity of urea peroxide was then added after completion of the reaction. The analyses of the products confirm that there are no substances in the tooth-whitening products tested which inhibit the activity of the catalase.
The same procedure was used on each of the products:
Arrow 1 indicates where tooth-whitening product and catalase were added
Arrow 2 indicates where more catalase was added
Arrow 3 indicates where urea peroxide solution in a known concentration was added.
3a: Product ”A”
3b: Product “B”
3c: Product “C”
3d: Product “D”
3e: Product “E” – first analysis (lot no. 30187)
3f: Product “E” – second analysis (lot no. 0704002)
3g: Product “F” – first analysis (lot no. 30187)
3h: Product “F” – second analysis (lot no. 0704002)
3i: Product “G”
Version 1.0 Marts 2009, © Danish Environmental Protection Agency