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Background Report on Pre-validation of an OECD Springtail Test Guideline

Contents

Summary and conclusions

Sammendrag og konklusioner

1 Introduction

• 1.1 Aims

2 Materials and methods

• 2.1 Background - species identification and comparison
  • 2.1.1 Literature search - Ecology and biology of species
• 2.2 Sensitivity comparison
  • 2.2.1 General sensitivity comparison
  • 2.2.2 Laboratory experiments - Comparison of age/size and toxicity
  • 2.2.3 Sex-ratio effects on offspring
• 2.3 Identifying test concentration ranges for ring-testing
  • 2.3.1 Test conditions

3 Results and Discussion

• 3.1 Background
  • 3.1.1 Ecology and Biology of Species
  • 3.1.2 Ecotoxicology
  • 3.1.3 Species inclusion in draft guideline
• 3.2 Sensitivity of species
  • 3.2.1 Ecotoxicology - General sensitivity differences between the two species
  • 3.2.2 Age- and size-toxicity relationships using dimethoate
  • 3.2.3 Additional differences: Sex-ratio of off-spring
• 3.3 Test concentration ranges of possible ring-test compounds
  • 3.3.1 Fipronil
  • 3.3.2 3,5-dichlorophenol
  • 3.3.3 Boric acid
  • 3.3.4 Proposed test ranges for a ring-test

4 Conclusion

5 References

OECD Guidelines for the Testing of Chemicals
Proposal for a New Guideline

• Annex 1
• Annex 2
• Annex 3
• Annex 4

List of participants
15 countries and 26 laboratories):

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Version 1.0 March 2005, © Danish Environmental Protection Agency