Karakterisering af havnesediment ved hjælp af biotest

Bilag I: Testresultater fra akut toksicitetstest med planktonalge (Skeletonema costatum)

Inhibition of the growth of Skeletonema costatum with "Frederiksholmløbet Kbh’s havn".

Statistical parameters calculated from continuous responses based on continuous mean.
Testtype : Growth Inhibition Test.
7 doses and 20 responses have been used during calculations.

Control values.

Concentration in mL/L

Growth

Inhibition in per cent

Control 1

0.103

-

Control 2

0.103

-

Control 3

0.103

-

Control 4

0.102

-

Control 5

0.104

-

Control 6

0.104

-

Control mean

0.103

0


Experimental Data.

Concentration in mL/L

Growth

Inhibition in per cent

5.0

0.097

6

5.0

0.097

6

5.0

0.097

6

10.0

0.092

11

10.0

0.091

12

10.0

0.092

11

20.0

0.082

21

20.0

0.082

21

20.0

0.083

20

50.0

0.066

36

50.0

0.065

37

50.0

0.069

33

100.0

0.052

50

100.0

0.052

50

100.0

0.056

46

200.0

0.032

69

200.0

0.033

68

200.0

0.035

66

500.0

0.026

75

500.0

0.018

83

500.0

0.000

100


Dunnett’s procedure:

NOEC: < 5.0 mL/L
LOEC: 5.0 mL/L

EC-values and limits of the 95% confidence interval.

y(EC)

LCL

EC(yo)

UCL

10

7.6

8.9

10.3

50

92.3

99.0

106.3

90

> 500.0


Inhibition of the growth of Skeletonema costatum with "Odense havn"

Statistical parameters calculated from continuous responses based on continuous mean.
Testtype : Growth Inhibition Test.
6 doses and 16 responses have been used during calculations.

Control values.

Concentration in mL/L

Growth

Inhibition in per cent

Control 1

0.103

-

Control 2

0.103

-

Control 3

0.103

-

Control 4

0.102

-

Control 5

0.104

-

Control 6

0.104

-

Control mean

0.103

0


Experimental Data.

Concentration in mL/L

Growth

Inhibition in per cent

5.0

0.104

0

5.0

0.106

0

5.0

0.104

0

10.0

0.104

0

10.0

0.105

0

10.0

0.103

0

20.0

0.103

0

20.0

0.102

1

20.0

0.102

1

50.0

0.099

4

50.0

0.097

6

50.0

0.097

6

100.0

0.093

10

100.0

0.092

11

100.0

0.093

10

200.0

0.084

19

200.0

0.086

17

200.0

0.084

19

500.0

0.075

27

500.0

0.076

26

500.0

0.072

30


Dunnett’s procedure:

NOEC: 20.0 mL/L
LOEC: 50.0 mL/L

EC-values and limits of the 95% confidence interval.

y(EC)

LCL

EC(yo)

UCL

10

81.6

95.2

108.3

50

> 500.0

90

> 500.0


Inhibition of the growth of Skeletonema costatum with "Horsens havn"

Statistical parameters calculated from continuous responses based on continuous mean.
Testtype : Growth Inhibition Test.
7 doses and 18 responses have been used during calculations.

Control values.

Concentration in mL/L

Growth

Inhibition in per cent

Control 1

0.102

-

Control 2

0.099

-

Control 3

0.102

-

Control 4

0.101

-

Control 5

0.100

-

Control mean

0.101

0


Experimental Data.

Concentration in mL/L

Growth

Inhibition in per cent

1.0

0.102

0

1.0

0.098

3

1.0

0.101

0

2.0

0.101

0

2.0

0.100

1

2.0

0.098

3

5.0

0.100

1

5.0

0.089

12

5.0

0.095

6

10.0

0.091

10

10.0

0.091

10

10.0

0.091

10

20.0

0.083

18

20.0

0.087

14

20.0

0.086

15

50.0

0.000

100

50.0

0.000

100

50.0

0.000

100

100.0

0.002

98

100.0

0.002

98

100.0

0.000

100


Dunnett’s procedure:

NOEC: 2.0 mL/L
LOEC: 5.0 mL/L

EC-values and limits of the 95% confidence interval.

y(EC)

LCL

EC(yo)

UCL

10

9.4

10.9

12.3

50

40.9

51.1

69.4

90

> 100.0


Inhibition of the growth of Skeletonema costatum with "Kattegat, station 905"

Statistical parameters calculated from continuous responses based on continuous mean.
Testtype : Growth Inhibition Test.
7 doses and 18 responses have been used during calculations.

Control values.

Concentration in mL/L

Growth

Inhibition in per cent

Control 1

0.103

-

Control 2

0.103

-

Control 3

0.103

-

Control 4

0.102

-

Control 5

0.104

-

Control 6

0.104

-

Control mean

0.103

0


Experimental Data.

Concentration in mL/L

Growth

Inhibition in per cent

5.0

0.105

0

5.0

0.104

0

5.0

0.103

0

10.0

0.104

0

10.0

0.102

1

10.0

0.101

2

20.0

0.102

1

20.0

0.101

2

20.0

0.100

3

50.0

0.100

3

50.0

0.098

5

50.0

0.096

7

100.0

0.095

8

100.0

0.095

8

100.0

0.094

9

200.0

0.087

16

200.0

0.088

15

200.0

0.090

13

500.0

0.055

47

500.0

0.071

31

500.0

0.052

50


Dunnett’s procedure:

NOEC: 20.0 mL/L
LOEC: 50.0 mL/L

EC-values and limits of the 95% confidence interval.

y(EC)

LCL

EC(yo)

UCL

10

79.9

111.4

140.6

50

> 500.0

90

> 500.0



Inhibition of the growth of Skeletonema costatum with " Ven kontrolsediment"

Statistical parameters calculated from continuous responses based on continuous mean.
Testtype : Growth Inhibition Test.
2 doses and 4 responses have been used during calculations.

Control values.

Concentration in mL/L

Growth

Inhibition in per cent

Control 1

0.104

-

Control 2

0.101

-

Control 3

0.104

-

Control 4

0.103

-

Control 5

0.102

-

Control mean

0.103

0


Experimental Data.

Concentration in mL/L

Growth

Inhibition in per cent

5.0

0.104

0

5.0

0.103

0

5.0

0.105

0

10.0

0.103

0

10.0

0.105

0

10.0

0.105

0

20.0

0.103

0

20.0

0.104

0

20.0

0.105

0

50.0

0.105

0

50.0

0.106

0

50.0

0.105

0

100.0

0.104

0

100.0

0.103

0

100.0

0.104

0

200.0

0.102

1

200.0

0.103

0

200.0

0.102

1

500.0

0.101

2

500.0

0.102

1


*) Data did not allow any statistical calculation of the EC-values.

Dunnett’s procedure:

NOEC: ³ 500.0 mL/L
LOEC: > 500.0 mL/L

 

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Inhibition of the growth of Skeletonema costatum with "3,5-DCP"

Date: 2000.11.14 - 2000.11.17

Statistical parameters calculated from continuous responses based on continuous mean.
Testtype : Growth Inhibition Test. Growth rate
6 doses and 12 responses have been used during calculations.

Control values.

Concentration in mg/L

Growth

Inhibition in per cent

Control 1

0.108

-

Control 2

0.107

-

Control 3

0.107

-

Control 4

0.109

-

Control 5

0.109

-

Control 6

0.108

-

Control mean

0.108

0


Experimental Data.

Concentration in mg/L

Growth

Inhibition in per cent

0.20

0.106

2

0.20

0.107

1

0.40

0.105

3

0.40

0.107

1

0.70

0.105

3

0.70

0.106

2

1.00

0.062

43

1.00

0.075

31

1.40

0.048

56

1.40

0.051

53

2.00

0.018

83

2.00

0.008

93


Dunnett’s procedure:

NOEC: 0.70 mg/L
LOEC: 1.00 mg/L

EC-values and limits of the 95% confidence interval.

y(EC)

LCL

EC(yo)

UCL

10

0.46

0.63

0.75

50

1.14

1.24

1.35

90

>2.00