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Alternatives to animal experiments for eye irritation

Preface

The Danish Environmental Protection Agency has sponsored the project "Validation of a three-dimensional model for eye and skin irritation". The study has been conducted as a joint venture between the Danish Environmental Protection Agency and the Danish Veterinary and Food Administration, Institute of Food Safety and Toxicology. The studies with the tissue model SKIN2 ZK1200 have been published in 4 articles that are the basis of the present report:

Brantom, P. G. et al.: A summary report of the COLIPA international validation study on alternatives to the Draize rabbit eye irritation test. Toxicology in Vitro, 1997, 11, 141-179.

Espersen, R. J., Olsen, P., Nicolaisen, G., Jensen, B. L. and Rasmussen, E. S.: Assessment of recovery from ocular irritancy using a human tissue equivalent model. Toxicology in Vitro, 1997, 11, 81-88.

Rasmussen, E. S.: Use of reconstructed human tissue models in toxicological and pharmacological studies. Dansk Veterinærtidsskrift, 1/11, 1996, 943-948.

Southee, J. A., McPherson, J. P., Osborne, R., Carr, G. J. and Rasmussen, E. S.: The performance of the tissue equivalent assay using the SKIN2TM ZK1200 model in the COLIPA international validation study on alternatives to the Draize eye irritation test. Toxicology in Vitro, 1999, 13, 355-373.

The project has been followed by a steering group with the following members:

The Danish Environmental Protection Agency:
Lone Mikkelsen (former chairman)
Lars Nørgaard (present chairman)
Anne Marie Linderstrøm

The Danish Veterinary and Food Administration:
John Christian Larsen
Eva Selzer Rasmussen (project leader)

The project has been accomplished by a project group at the Institute of Food Safety and Toxicology with the following members:

René Espersen
Preben Olsen
Bo Lund Jensen
Gert Maarløw Nicolaisen
Martin Bach
Eva Selzer Rasmussen

The members of the steering group and the project gruop are acknowledged for their excellent collaboration.

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