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Alternatives to animal experiments for eye irritation
Colophon
Title
Alternatives to animal experiments for eye irritation
Author
Rasmussen, Eva Selzer
Publisher
221 Danish Environmental Protection Agency
Responsible institution
Fødevaredirektoratet, Institut for Fødevaresikkerhed og Toksikologi
Copyright
Ministry of Environment and Energy. Danish Environmental Protection Agency
Series and number
Working report 10/2000
Keywords
eng: cosmetics; eye irritation; in vitro tests; alternatives; animal testing
Emneord
dan: kosmetik; øjne; irritation; analysemetoder; dyreforsøg; vævskulturer; substitution
Abstract
eng: The goal of the project was to evaluate the prospect for the replacement of rabbit
eye irritation studies with in vitro tests. A corneal tissue equivalent model, SKIN2 ZK
1200, was used to evaluate 55 cosmetic ingredients and finished products. The study was a
part of a large validation study arranged by the european cosmetics industry (COLIPA). The
tissue model performed very well in the prediction of acute eye irritation in rabbit
experiments, both for ingredients and finished products. A new testing procedure developed
with the tissue model showed also promise for prediction of recovery from ocular
irritation.
Resumé
dan: Formålet med projektet var at undersøge, om in vitro tests kan erstatte forsøg for
øjenirritation med kaniner. Testningen indgik i en stor valideringsundersøgelse
arrangeret af den europæiske kosmetikindustri (COLIPA). En vævsmodel for øjets
hornhinde, SKIN2 ZK 1200, blev anvendt til at bedømme 55 kosmetiske indholdsstoffer og
færdige produkter. Modellen var meget god til forudsigelse af akut øjenirritation i
kaninforsøg, både for ingredienser og færdige produkter. Der blev tillige udviklet en
test, der kan forudsige vedvarende øjenirritation.
Language
eng
ISBN - electronic
ISBN 87-7909-095-9
Version
1.0
Version date
2000.06.06
Format
htm; gif; jpg
Inventory
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Publication standard no.
1.0
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